Some applications of high-performance liquid chromatography in the field of nucleotides, nucleosides and bases.

It is difficult to speak solely about recent developments in a field such as the chromatography of nucleotides, nucleosides and bases since their separation was among the first applications of liquid column chromatography on ionexchange resins (Cohn, 1949). This methodology was subsequently refined, improved and applied to a variety of biochemical and clinical problems by many authors. It was to these same compounds that the first applications of modern h.p.1.c. were made when Horvath et al. (1967) developed pellicular ion-exchangers, the first high-efficiency column-packing materials.